PHOSPHATIDIC ACID PHOSPHATASE HOMOLOGUES AND THEIR APPLICATION Russian patent published in 2014 - IPC C12N15/55 C12N9/16 C12N1/19 C12N1/15 C12P7/64

Abstract RU 2507264 C2

FIELD: biotechnologies.

SUBSTANCE: from Mortierella alpina a new gene was obtained coding phosphatidic acid phosphatase (EC 3.1.3.4), open reading frame was determined and amino acid sequence of enzyme, named as MaPAP1, was determined. Method for obtainment of recombinant protein by transformation of host cells was described, where upon use of yeast cells or cells of lipid producing fungi as host cells not only increase of total quantity of fatty acids, but change of fatty acid composition cell is provided as well.

EFFECT: increase of arachidonic acid quantity relation to total quantity of fatty acids.

8 cl, 7 tbl, 2 dwg, 8 ex

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RU 2 507 264 C2

Authors

Otiai Misa

Tokuda Khisanori

Dates

2014-02-20—Published

2008-07-10—Filed