FIELD: biotechnology, molecular biology, genetic engineering. SUBSTANCE: invention relates to development of new recombinant plasmids expressing fused protein where amino acid sequences of lider peptide that is IgG-binding domain of protein A and human proinsulin are bound by two arginine residues (plasmid pRRproins) or lysine and arginine residues (plasmid pRRproins). Human insulin is prepared by a method that involves culturing the strain E. coli JM-109-pRRproins carrying plasmid pRRproins or the strain E. coli JM-109-pRRproins carrying plasmid pRRproins, isolation of inclusion bodies, solubilization, sulfitolysis and renaturation of fused protein by ion-exchange chromatography method, its cleavage with trypsin-like and carboxypeptidase B-like enzymes and insulin isolation. EFFECT: simplified technological process of human insulin preparing, increased yield of insulin. 6 cl, 4 dwg, 7 ex
Title |
Year |
Author |
Number |
RECOMBINANT PLASMID ENCODING FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), METHOD OF HUMAN INSULIN PREPARING |
1998 |
- Navashin S.M.
- Mal'Tsev K.V.
- Jarotskij S.V.
|
RU2144082C1 |
RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN |
2006 |
- Shmatchenko Vadim Vasil'Evich
- Shmatchenko Natal'Ja Anatol'Evna
- Bajdus' Aleksandr Nikolaevich
- Kuptsov Vasilij Nikolaevich
- Nozdrin Viktor Nikolaevich
- Stepanov Aleksej Vjacheslavovich
|
RU2354702C2 |
RECOMBINANT PLASMID DNA pMSIN4, CODING HYBRIDE POLYPEPTIDE - HUMAN INSULIN PRECURSOR, BL21(DE3)VpMSIN4-PRODUCER STRAIN OF RECOMBINANT HUMAN INSULIN, METHOD FOR PRODUCING RECOMBINANT HUMAN INSULIN |
2011 |
- Kostetskij Igor' Evgen'Evich
- Lisovskij Igor' Leonidovich
- Lutsiv Vladimir Romanovich
- Markeeva Natal'Ja Vladimirovna
|
RU2447149C1 |
METHOD OF PREPARING STRAIN-PRODUCER OF FUSED PROTEIN APROINS FOR HUMAN INSULIN PREPARING |
1998 |
- Markin S.S.(Ru)
- Uvarov V.Ju.(Ru)
- Ljashenko A.A.(Ru)
- Smirnova E.G.(Ru)
- Semsnov M.P.(Ru)
- Borshchenko Natal'Ja Jur'Evna
- Tarasov Aleksandr Andreevich
|
RU2148641C1 |
STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN |
2007 |
- Rodionov Petr Ivanovich
- Rodionov Petr Petrovich
- Shmatchenko Vadim Vasil'Evich
- Stepanov Aleksej Vjacheslavovich
- Bajdus' Aleksandr Nikolaevich
- Shmatchenko Natal'Ja Anatol'Evna
- Gorkun Taras Alekseevich
|
RU2376368C2 |
RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2515115C1 |
METHOD OF RECOMBINANT HUMAN INSULIN PREPARING |
1999 |
- Kalinin Ju.T.
- Urakov N.N.
- Ivanov V.T.
- Stepanov A.V.
- Miroshnikov A.I.
- Gavrikov V.G.
- Bairamashvili D.I.
- Donetskij I.A.
- Korobko V.G.
- Kostromina T.I.
- Guljaev V.A.
- Litvinenko M.A.
- Bajdus' A.N.
- Korobov V.I.
- Krasil'Nikov V.A.
- Borisov N.V.
- Shmatchenko N.A.
- Vorotnikova I.I.
- Gorkun O.G.
- Derbyshev V.I.
- Nozdrin V.N.
- Dunajtsev I.A.
- Lipin M.Ju.
- Bekmuratova I.I.
- Kobeleva V.A.
- Shepelin A.P.
- Martovetskij M.N.
- Fedjukin V.S.
- Abrosimova L.I.
|
RU2141531C1 |
RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
RECOMBINANT PLASMID DNA PF265 CODING HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2728611C1 |