FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce recombinant human proinsulin. Recombinant proinsulin is obtained in a hybrid polypeptide, in which the human HGS protein fragment sequence, substrate of tyrosine kinase is connected through peptide linker with sequence GlyHis6GlySerArg with amino acid sequence of human proinsulin, with arginine as site of proteolysis between C-peptide and chain A. For this purpose, recombinant plasmid DNA pF265 and strain-producer Escherichia coli BL21/pF265 is used.
EFFECT: invention provides synthesis of a hybrid polypeptide with an expression level of not less than 4_5 % of the crude weight of the cell biomass, increases the portion of insulin in the hybrid protein to 49_5 % and increases efficiency of purifying the end product by eliminating the closely related Arg0-A-insulin admixture.
3 cl, 5 dwg, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PF267 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN LISPRUM, AND A STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN LISPRO |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729357C1 |
| RECOMBINANT PLASMID DNA PF644 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN GLARGINE, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN GLARGINE |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729381C1 |
| RECOMBINANT PLASMID DNA PF646 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN ASPART, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN ASPART |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729353C1 |
| RECOMBINANT PLASMID DNA OF PF882, WHICH CODES HYBRID POLYPEPTIDE CONTAINING B30-DESTHREONINE-INSULIN, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING 30-DESTHREONINE-INSULIN |
2019 |
- Latypov Vitalii Feliksovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729737C1 |
| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
- Gavrikov V.G.
- Kalinin Ju.T.
- Stepanov A.V.
|
RU2144957C1 |
| RECOMBINANT PLASMID DNA PPINS16 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN, RECOMBINANT PLASMID DNA PPINS25 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Miroshnikov A.I.
- Gavrikov V.G.
- Stepanov A.V.
- Kalinin Ju.T.
|
RU2143493C1 |
| RECOMBINANT PLASMID DNA pHINSO5 ENCODING HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN, ESHERICHIA COLI CELL TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS05 AND STRAIN OF BACTERIUM ESCHERICHIA COLI JM109/pHINS05 AS PRODUCER OF HYBRID POLYPEPTIDE COMPRISING HUMAN PROINSULIN |
2004 |
- Shmatchenko V.V.
- Piskareva O.A.
- Shmatchenko N.A.
- Bajdus' A.N.
- Stepanov A.V.
|
RU2263147C1 |
| RECOMBINANT PLASMID ENCODING FUSED PROTEIN WITH HUMAN PROINSULIN (VARIANTS) |
1999 |
|
RU2162102C1 |
| RECOMBINANT PLASMID DNA pHILP07 ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHILP07, AND STRAIN OF BACTERIA Escherichia coli JM109/pHILP07 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2514480C1 |
| RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
