FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology, particularly genetic and protein engineering, as well as a method of producing recombinant human C-peptide. The disclosed method involves culturing an Escherichia coli producing strain, breaking down bacterial cells by disintegration, separating "inclusion bodies" containing a hybrid protein, dissolution thereof in a buffer containing urea and dithiothreitol, renaturating and purifying the renatured hybrid protein, disintegration thereof with trypsin and carboxypeptidase B, separation via chromatography on SP-sepharose, followed by purification and obtaining the end product.
EFFECT: method enables to obtain C-peptide with high output and purity of not less than 95% from wastes formed when producing recombinant human insulin.
4 tbl, 6 ex
| Title | Year | Author | Number |
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| METHOD FOR INDUSTRIAL PREPARING HUMAN RECOMBINANT INSULIN | 2003 |
|
RU2232813C1 |
| RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN | 2006 |
|
RU2354702C2 |
| METHOD OF RECOMBINANT HUMAN INSULIN PREPARING | 1999 |
|
RU2141531C1 |
| STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN | 2007 |
|
RU2376368C2 |
| METHOD FOR PREPARING GENETIC-ENGINEERING HUMAN INSULIN | 2006 |
|
RU2322504C1 |
| RECOMBINANT PLASMID DNA pMSIN4, CODING HYBRIDE POLYPEPTIDE - HUMAN INSULIN PRECURSOR, BL21(DE3)VpMSIN4-PRODUCER STRAIN OF RECOMBINANT HUMAN INSULIN, METHOD FOR PRODUCING RECOMBINANT HUMAN INSULIN | 2011 |
|
RU2447149C1 |
| RECOMBINANT PLASMID ENCODING FUSED PROTEIN AS PRECURSOR OF HUMAN INSULIN (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED PROTEIN AS PRECURSOR OF HUMAN INSULIN (VARIANTS), METHOD OF HUMAN INSULIN PREPARING | 1998 |
|
RU2143492C1 |
| RECOMBINANT PLASMID ENCODING FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED PROTEIN-PRECURSOR OF HUMAN INSULIN (VARIANTS), METHOD OF HUMAN INSULIN PREPARING | 1998 |
|
RU2144082C1 |
| CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES | 1997 |
|
RU2122549C1 |
| STRAIN ESCHERICHIA COLI XLI - BLUE/PINSR - A PRODUCER OF PREPROINSULIN | 1997 |
|
RU2122581C1 |
