METHOD FOR PREPARING PREPARATION FORTELYSIN SHOWING FIBRINOLYTIC PROPERTIES, AND ITS APPLICATION FOR TREATING MYOCARDIAL INFRACTION Russian patent published in 2012 - IPC C12N9/14 A61K39/108 A61P9/00 

FIELD: medicine.

SUBSTANCE: supernatant containing a recombinant protein with an amino acid sequence of staphylokinase (RPSF) is defrozen. The RPSF is recovered and chromatographically purified on CM-sepharose. The RPSF is chromatographically purified on DEAE-sepharose. Then the RPSF is transferred into 0.3% NaCl solution. It is followed by sterilisation, packaging, lyophilisation and marking of the substance. 20 litres of water for injections are introduced in a stirred reactor 160 l, then 150 g of the substance and 10 l of water for injections are added; the stirrer is actuated (stirring rate is 80 rpm); the stirring procedure is carried out for 15 min; thereafter the stirrer is stopped; the substance is sampled; the pH value of the prepared solution is recorded; pH is 5.5 to 7.5. It is followed by sterilisation filtration of the solution, filling and pre-corking of bottles. Then lyophilisation and final corking are performed. What is offered is the application of the preparation Fortelysin for treating myocardial infraction by the intravenous introduction in dose 15 mg (2235000 IU) with the content of a bottle 5 mg (745000 IU) before the introduction of the preparation is dissolved in 0.9% sodium chloride 5 ml; the preparation is introduced in two boluses 10 mg (1490000 IU) simultaneously and in 30 minutes 5 mg (745000 IU) or at first 10 mg (1490000 IU) in boluses, and the rest 5 mg (745000 IU) in intravenous infusions in 0.9% sodium chloride 50 ml for 30 min.

EFFECT: invention extends the range of drug preparations with fibrinolytic properties.

3 cl