PREPARATION FOR TREATMENT OF MUSCULAR DYSTONIA FROM TOXIN OF CULTURE CLOSTRIDIUM BOTULINUM AND METHOD FOR ITS PREPARING Russian patent published in 2005 - IPC

FIELD: medicine, microbiology, pharmacology.

SUBSTANCE: invention proposes a medicinal preparation for treatment of muscular dystonia that comprises botulinic toxin isolated from culture Clostridium botulinum as a liquid substance dissolved mainly in 0.87% physiological solution with albumin. Method for preparing the preparation involves preparing botulinic toxin from culture Clostridium botulinum by culturing its strain at temperature 29-33°C for 3-6 days in nutrient medium. Then dry ammonium sulfate is added to cultural fluid slowly for formation of precipitate. Prepared suspension is centrifuged for preparing precipitate and isolated liquid phase is extracted and subjected for purification. Process for purifying the liquid substance of medicinal preparation is carried out for two stages using metal sorbent at the first stage and gel-filtration at pH 5.5-6.0 - at the second stage. Sterilization of the purified substance is carried out by membrane filtration. Invention provides preparing the preparation with reduced risk for arising intolerance and with high stability in storage.

EFFECT: improved method for preparing, valuable medicinal properties of preparation.

6 cl, 7 ex