METHOD OF PRODUCTION AND PREPARATION OF ANALOGUE OF HUMAN RECOMBINANT INTERFERON GAMMA Russian patent published in 2008 - IPC C12N15/23 C12P21/02 A61K38/21 A61P35/00 

Abstract RU 2326944 C2

FIELD: technological processes; medicine.

SUBSTANCE: invention is related to preparation of recombinant analogues of human gamma-interferon and may be used in medicine for prophylactics and treatment of oncological diseases, neoplasms and inflammatory processes of humans. Highly pure genetically engineered analogue of human gamma-interferon - deltaferon with molecular mass of 16.2 kilo Daltons is produced by microbiological synthesis with further chromatographic purification on "KM"-sepharose - cationic-exchange sorbent with high linear speed of flow and suitable for preparative loads, at different pH values. On the basis of deltaferon, which contains by data of polyacrylamide gel-sodium dodecyl sulfate electrophoresis at least 98% of main substance in the form of monomer that possesses antiproliferative and anti-inflammatory activities inherent in gamma-interferon, medical product is prepared, which also includes low molecular polymer filler-stabiliser (reopolyglukine or polyvinylpyrrolidone) and salt buffer system with pH 7.0-7.1.

EFFECT: highly active and stable preparation of deltaferon is produced.

2 cl, 6 dwg, 1 tbl, 3 ex

Similar patents RU2326944C2

Title Year Author Number
METHOD OF HUMAN RECOMBINANT TUMOR NECROSIS ALPHA-FACTOR PREPARING 1997
  • Pustoshilova N.M.
  • Kileva E.V.
  • Denisova L.Ja.
  • Shingareva N.V.
  • Korobko V.G.
  • Denisov L.A.
  • Masycheva V.I.
  • Sandakhchiev L.S.
  • Kalinin Ju.T.
RU2144958C1
METHOD OF HUMAN RECOMBINANT GAMMA-INTERFERON PREPARING 1997
  • Zakabunin A.I.
  • Baranovskaja G.A.
  • Pustoshilova N.M.
  • Majstrenko V.F.
  • Gavrjuchenkova L.P.
  • Gromova O.A.
RU2132386C1
RECOMBINANT PLASMID DNA p280_2GM CODING POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN, STRAIN ESCHERICHIA COLI SG 20050/p280_2GM - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR OF HUMAN AND METHOD OF OBTAINING OF SAID POLYPEPTIDE 2018
  • Gileva Irina Pavlovna
  • Esina Tatyana Igorevna
  • Volosnikova Ekaterina Aleksandrovna
  • Gogina Yana Stanislavovna
  • Lebedev Leonid Rudolfovich
  • Danilenko Elena Dmitrievna
RU2708556C1
METHOD FOR PREPARING HIGHLY PURIFIED RECOMBINANT POLYPEPTIDE WITH PROPERTY OF HUMAN TUMOR NECROSIS FACTOR-ALPHA 2004
  • Pustoshilova Nina Mikhajlovna
  • Zakabunin Aleksandr Ivanovich
  • Kozlova Julija Nikolaevna
  • Afinogenova Galina Nikolaevna
  • Kan'Shina Angelina Vasil'Evna
  • Litovchenko Lidija Leonidovna
RU2274656C1
RECOMBINANT PLASMIDS pET32a-IFG144, PROVIDING INTERFERON GAMMA SYNTHESIS, BACTERIAL STRAIN ESCHERICHIA COLI JM109/pET32a-IFG144 - INTERFERON GAMMA PROTEIN PRODUCER, METHOD OF OBTAINING INTERFERON GAMMA AND MEDICINAL PRODUCT 2022
  • Chumburidze Georgij Georgievich
RU2809358C1
RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN 2006
  • Shmatchenko Vadim Vasil'Evich
  • Shmatchenko Natal'Ja Anatol'Evna
  • Bajdus' Aleksandr Nikolaevich
  • Kuptsov Vasilij Nikolaevich
  • Nozdrin Viktor Nikolaevich
  • Stepanov Aleksej Vjacheslavovich
RU2354702C2
RECOMBINANT PLASMIDS pET32a-IFG144, PROVIDING INTERFERON GAMMA SYNTHESIS, BACTERIAL STRAIN ESCHERICHIA COLI JM109/pET32a-IFG144 - INTERFERON GAMMA PROTEIN PRODUCER, METHOD OF OBTAINING INTERFERON GAMMA AND MEDICINAL PRODUCT 2022
  • Chumburidze Georgij Georgievich
RU2809360C1
METHOD OF PREPARING POLYPEPTIDE SHOWING HUMAN LYMPHOTOXIN PROPERTY 1992
  • Pustoshilova N.M.
  • Lebedev L.R.
  • Gileva I.P.
  • Korobko V.G.
  • Davydov I.V.
  • Petrenko V.A.
RU2048521C1
METHOD FOR MANUFACTURING HUMAN RECOMBINATION LYMPHOTOXIN 1992
  • Denisov A.A.
  • Torskij S.P.
  • Nikolaeva O.G.
  • Volozhantseva I.Ja.
  • Urakov N.N.
  • Korobko V.G.
RU2039823C1
METHOD FOR INDUSTRIAL PREPARING HUMAN RECOMBINANT INSULIN 2003
  • Kalinin Ju.T.
  • Stepanov A.V.
  • Bajdus' A.N.
  • Nozdrin V.N.
  • Aitov R.S.
  • Kolesnichenko I.F.
  • Shmatchenko N.A.
  • Babaev M.A.
  • Borisov N.V.
  • Krasil'Nikov V.A.
  • Gorkun O.G.
  • Astashkina G.F.
  • Ushakova N.I.
RU2232813C1

RU 2 326 944 C2

Authors

Miroshnikov Pavel Nikolaevich

Lebedev Leonid Rudol'Fovich

Masycheva Valentina Ivanovna

Dates

2008-06-20Published

2006-08-14Filed