FIELD: technological processes; medicine.
SUBSTANCE: invention is related to preparation of recombinant analogues of human gamma-interferon and may be used in medicine for prophylactics and treatment of oncological diseases, neoplasms and inflammatory processes of humans. Highly pure genetically engineered analogue of human gamma-interferon - deltaferon with molecular mass of 16.2 kilo Daltons is produced by microbiological synthesis with further chromatographic purification on "KM"-sepharose - cationic-exchange sorbent with high linear speed of flow and suitable for preparative loads, at different pH values. On the basis of deltaferon, which contains by data of polyacrylamide gel-sodium dodecyl sulfate electrophoresis at least 98% of main substance in the form of monomer that possesses antiproliferative and anti-inflammatory activities inherent in gamma-interferon, medical product is prepared, which also includes low molecular polymer filler-stabiliser (reopolyglukine or polyvinylpyrrolidone) and salt buffer system with pH 7.0-7.1.
EFFECT: highly active and stable preparation of deltaferon is produced.
2 cl, 6 dwg, 1 tbl, 3 ex
| Title |
Year |
Author |
Number |
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- Pustoshilova N.M.
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- Denisov L.A.
- Masycheva V.I.
- Sandakhchiev L.S.
- Kalinin Ju.T.
|
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- Gromova O.A.
|
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|
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|
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- Pustoshilova N.M.
- Lebedev L.R.
- Gileva I.P.
- Korobko V.G.
- Davydov I.V.
- Petrenko V.A.
|
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- Denisov A.A.
- Torskij S.P.
- Nikolaeva O.G.
- Volozhantseva I.Ja.
- Urakov N.N.
- Korobko V.G.
|
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- Kalinin Ju.T.
- Stepanov A.V.
- Bajdus' A.N.
- Nozdrin V.N.
- Aitov R.S.
- Kolesnichenko I.F.
- Shmatchenko N.A.
- Babaev M.A.
- Borisov N.V.
- Krasil'Nikov V.A.
- Gorkun O.G.
- Astashkina G.F.
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|
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