MEDICINAL PREPARATION FOR TREATMENT OF MUSCLE DYSTONIA AND METHOD FOR ITS PREPARING Russian patent published in 2003 - IPC

FIELD: medicine, microbiology, pharmacy. SUBSTANCE: medicinal preparation comprises botulinic toxin isolated from culture Clostridium botulinum type A, strain = 98, as a liquid substance dissolved mainly in 0.87% 0.04 M solution of sodium chloride, pH 4.9-6.9. Method for preparing medicinal preparation involves preparing botulinic toxin type A from culture Clostridium botulinum, strain = 98, by its culturing at temperature 29-30 C for 3-6 days in nutrient medium. Then dry ammonium sulfate is added slowly to cultural fluid to form precipitate, prepared suspension is centrifuged to isolate precipitate and isolated liquid phase is extracted and subjected for purification. Process of purification of liquid substance is carried out for two stages where at the first step nickel-base metallosorbent is used mainly and at the second step ion-exchange sorbent at pH 6.1-6.9 is used. Sterilization of purified substance is carried out by membrane filtration. Invention provides preparing the preparation no losing activity in liquid form. EFFECT: improved preparing method, valuable medicinal properties of preparation. 3 cl, 1 ex