FIELD: microbiology.
SUBSTANCE: method involves culturing an indicator strain on solid nutrient medium and the following estimation of antagonistic activity. Analyzed strain is cultured in combination with mycobacterial strain on Soton's medium followed by preparing the cultural fluid sterile filtrate. Filtrated is stratified on the solid nutrient medium surface before seeding an indicator strain. Antagonistic activity is evaluated by the ratio of amount of indicator strain colonies on solid nutrient medium to the amount of indicator strain colonies on solid nutrient medium with a stratified sterile filtrate of cultural fluid after their culturing analyzed and mycobacterial strains. Method provides detecting antagonistic properties with respect to pathogenic microorganisms in lactic acid microorganisms and microorganisms of Escherichia coli group.
EFFECT: improved detecting method.
4 tbl, 5 ex
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METHOD FOR EVALUATING ANTAGONISTIC ACTIVITY OF LACTIC-ACID BACTERIA OR ENTEROBACTERIA TO PATHOGENIC MYCOBACTERIA DUE TO APPLYING A TWO-LAYER MEDIUM | 2006 |
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DENSE GROWTH MEDIUM FOR CULTIVATION OF PATHOGENIC MYCOBACTERIA AND NOCARDIOFORM RAY FUNGI | 2008 |
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METHOD OF SPECIFIC DIFFERENTIATION OF TUBERCULOSIS MYCOBACTERIA | 2009 |
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Authors
Dates
2008-03-27—Published
2006-10-18—Filed