ENZYMATIC DEMETHYLATION OF FLAVONOIDS Russian patent published in 2012 - IPC C12P17/02 C12N1/20 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. The method of producing 8-prenylnaringenin in vitro involves: a) providing a first composition containing a culture of Eubacterium limosum or Peptostreptococcus productus bacterial cells, having 5-alkoxyflavonoid-dealkylating activity, and b) bringing a second composition containing 5-alkoxyflavonoids into contact with said first composition to facilitate dealkylation of said 5-alkoxyflavonoids with said bacterial cell culture. Disclosed is a combination for obtaining 8-prenylnaringenin in vitro and use of the culture of Eubacterium limosum or Peptostreptococcus productus bacterial cells, having 5-alkoxyflavonoid-dealkylating activity to obtain 8-prenylnaringenin in vitro. The method of enhancing 5-alkoxyflavonoid-dealkylating activity of the culture of Eubacterium limosum or Peptostreptococcus productus bacterial cells involves a step for inoculating said bacterial cell culture on a medium containing 5-alkoxyflavonoids, and a step for selecting a colony with the highest 5-alkoxyflavonoid-dealkylating activity. The Eubacterium limosum LMG P-23546 strain has 5-alkoxyflavonoid-dealkylating activity and is capable for converting isoxanthohumol to 8-prenylnaringenin.

EFFECT: invention increases efficiency of producing a phytoextractant such as 8-prenylnaringenin.

14 cl, 9 dwg, 4 tbl, 6 ex