FIELD: medicine.
SUBSTANCE: pieces of the affected organs (a biomaterial) are grinded to prepare a suspension. The prepared suspension is filled with a solution containing 4-6% of citric acid and 3% of hydrogen peroxide in the ratio 1:1 to the biomaterial suspension and kept for 30-60 minutes at room temperature to be thereafter processed in a solution containing 4-5% of succinic acid and 3% of hydrogen peroxide in the ratio to the suspension 1:1. It is kept for 30-60 minutes and then neutralised with 5-10% ammonia to pH 7.0. It is followed by decantation of the supernatant, and the Lowenstein-Jensen agar medium is inoculated with the precipitation.
EFFECT: invention allows reducing mycobacteria recovery time.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR RECOVERING PURE STAPHYLOCOCCUS CULTURE | 2011 |
|
RU2458990C1 |
| NUTRIENT MEDIUM FOR ISOLATION AND CULTURING MYCOBACTERIA FROM BIOLOGICAL MATERIAL | 2006 |
|
RU2315812C1 |
| METHOD FOR CULTIVATION OF TUBERCULOSIS MYCOBACTERIA | 2008 |
|
RU2376365C1 |
| METHOD OF PRE-SEEDING TREATMENT OF PATHOLOGICAL MATERIAL FOR EXTRACTION OF NOCARDIOFORMAL ACTINOMYCETES | 2014 |
|
RU2559523C1 |
| METHOD OF DETECTING MYCOBACTERIUM TUBERCULOSIS IN AIR ENVIRONMENT | 2011 |
|
RU2490328C2 |
| METHOD FOR PRE-INOCULATION PROCESSING OF PATHOLOGICAL MATERIAL FOR MYCOBACTERIAL RECOVERY | 2013 |
|
RU2542460C1 |
| METHOD FOR MYCOBACTERIA DETECTION FROM SURFACES | 2016 |
|
RU2619220C1 |
| METHOD OF PRE-INOCULATION PROCESSING OF SAMPLES COLLECTED FROM ENVIRONMENT OBJECTS FOR RELEASE OF MICROBACTERIA | 2009 |
|
RU2402781C1 |
| METHOD FOR DETERMINATION OF EFFICIENCY OF DISINFECTANTS USED IN TB FACILITIES | 2008 |
|
RU2364629C1 |
| SYNTHETIC NUTRIENT MEDIUM FOR GROWING MICROORGANISMS | 2010 |
|
RU2439142C1 |
