FIELD: medicine.
SUBSTANCE: milled samples of biological material are poured with physiological solution for 24 hours at room temperature, filtered through wadding, centrifuged for 20 min at 3-4 thousand revolutions per minute. After that 1.5% solution of lauryl sulphate per 1.5% solution of caustic soda is added to sediment, kept for 20 minutes, centrifuged for 20 minutes at 3-4 thousand revolutions per minute, sediment is washed with physiological solution 2-3 times and inoculated on nutrient medium.
EFFECT: application of method allows to optimise pre-inoculation processing of biomaterial, and increase efficiency of bacteriological diagnostics of tuberculosis.
1 ex, 1 tbl
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Authors
Dates
2010-10-27—Published
2009-06-23—Filed