FIELD: chemistry.
SUBSTANCE: invention relates to microbiology and is meant for detecting mycobacterium tuberculosis in the air environment of prevention and treatment facilities. The method involves collecting air samples through directed impact action of a jet on a foam plate in a Petri dish, said plate being soaked in 4-6 ml of 5% trisubstituted sodium phosphate solution followed by incubation of the Petri dish wit the foam plate for 18-24 hours; centrifuging the analysed material, followed by separation of supernatant fluid and neutralising the precipitate with 1% citric acid solution in ratio of 1:1, followed by inoculating the neutralised precipitate on a Novaya culture medium and culturing until growth. The diameter of the foam plate is equal to the diameter of the bottom of the Petri dish and its thickness is 4-6 mm.
EFFECT: invention increases reliability of detecting mycobacteria in an air environment.
4 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD OF DETECTION OF MYCOBACTERIUM FROM SURFACE | 1999 |
|
RU2180690C2 |
METHOD FOR DETECTING OF MYCOBACTERIA FROM SURFACES | 2008 |
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RU2415945C2 |
METHOD FOR ISOLATING TUBERCULOSIS MYCOBACTERIA FROM AIR MEDIUM | 2001 |
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RU2221047C2 |
METHOD FOR DETERMINATION OF EFFICIENCY OF DISINFECTANTS USED IN TB FACILITIES | 2008 |
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RU2364629C1 |
METHOD OF DETECTION OF MYCOBACTERIA | 2001 |
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RU2203957C2 |
METHOD FOR MYCOBACTERIA DETECTION FROM SURFACES | 2016 |
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RU2619220C1 |
METHOD OF DETECTION OF TUBERCULOSIS MYCOBACTERIA | 1993 |
|
RU2069698C1 |
METHOD FOR REVEALING CATTLE TUBERCULOSIS MYCOBACTERIA | 2006 |
|
RU2328526C1 |
METHOD FOR BACTERIOLOGICAL DETECTION OF NOCARDIOMORPHOUS ACTINOMYCES | 2006 |
|
RU2321636C1 |
METHOD FOR DETECTION OF TUBERCULOSIS MYCOBACTERIA IN URINE | 2002 |
|
RU2241041C2 |
Authors
Dates
2013-08-20—Published
2011-06-29—Filed