METHOD OF DETECTING MYCOBACTERIUM TUBERCULOSIS IN AIR ENVIRONMENT Russian patent published in 2013 - IPC C12Q1/04 C12N1/20 

Abstract RU 2490328 C2

FIELD: chemistry.

SUBSTANCE: invention relates to microbiology and is meant for detecting mycobacterium tuberculosis in the air environment of prevention and treatment facilities. The method involves collecting air samples through directed impact action of a jet on a foam plate in a Petri dish, said plate being soaked in 4-6 ml of 5% trisubstituted sodium phosphate solution followed by incubation of the Petri dish wit the foam plate for 18-24 hours; centrifuging the analysed material, followed by separation of supernatant fluid and neutralising the precipitate with 1% citric acid solution in ratio of 1:1, followed by inoculating the neutralised precipitate on a Novaya culture medium and culturing until growth. The diameter of the foam plate is equal to the diameter of the bottom of the Petri dish and its thickness is 4-6 mm.

EFFECT: invention increases reliability of detecting mycobacteria in an air environment.

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RU 2 490 328 C2

Authors

Mordovskoj Georgij Georgievich

Golubev Dmitrij Nikolaevich

Egorova Oksana Stepanovna

Dates

2013-08-20Published

2011-06-29Filed