METHOD FOR SIMULATING ACTION OF DIFFERENT CONCENTRATIONS OF OZONE-OXYGEN MIXTURE INTRODUCED IN ABDOMINAL CAVITY IN EXPERIMENT Russian patent published in 2012 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: anaesthesia care and an abdominal puncture are followed by introducing a clamped catheter tightly whereto a mechanical or electronic mercury-scale manometer and a Janet's syringe filled with the ozone-oxygen mixture are attached by two short clamped tubes. It is followed by measuring the intracavitary pressure with using the manometer and opening the clamps on the catheter and the tube attached to the manometer. It is followed by opening the clamp of the tube attached to the Janet's syringe and introducing the ozone-oxygen mixture in the concentrations 40, 60, 80 and 100 mg/l slowly inside the abdominal cavity. While introducing the ozone-oxygen mixture, the pressure indications are monitored on the manometer with the abdominal pressure being set to the value exceeding a reference by 1 mm Hg exactly. Thereafter, the introduction is stopped, and the manometer indication accuracy is additionally checked with the clamp of the tube attached to the Janet's syringe closed herewith introducing another volume of the ozone-oxygen mixture into the abdominal cavity or supplying an excess volume back to the Janet's syringe if required. Then the catheter is tightly removed from the abdominal cavity with the ozone-oxygen mixture kept therein. The animals are removed from the experiment in 4 hours, on the 1, 3, 7 and 30 day for each of the studied concentrations with the intra- and extracavitary tissue and organ structures to be examined at a macro- and microscopic level. Also, safety and toxicity issues are considered for the studied concentrations of the ozone-oxygen mixture.

EFFECT: method enables the most accurate and complete assessment of action of the concentrations of the ozone-oxygen mixture when introduced in the animal's abdominal cavity ensured by individual selection and control of the introduced volume of the mixture required to be distributed in the abdominal cavity completely in the normal compression environment, as well as provides detecting the safety and toxicity ranges of the concentrations of the ozone-oxygen mixture.