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2 463 347

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IPC

C12N15/00(2006-01-01)

C12N15/11(2006-01-01)

(21) (22)

Application

2010121694/10, 2010-05-28

(24)

Start date

2010-05-28

(22)

Actual filing date

2010-05-28

(45)

Published

2012-10-10

(72)

Inventor

Chuvilin Andrej NikolaevichPozmogova Galina Evgen'EvnaSmirnov Igor' PavlovichGovorun Vadim Markovich

(73)

Holder

Chuvilin Andrej NikolaevichPozmogova Galina Evgen'EvnaSmirnov Igor' PavlovichGovorun Vadim MarkovichFederal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauki Institut Fiziko-Khimicheskoj Meditsiny Federal'Nogo Mediko-Biologicheskogo Agentstva"

METHOD FOR PRODUCING HEXACHLOROFLUORESCEINE-MARKED OLIGONUCLEOTIDES Russian patent published in 2012 - IPC C12N15/00 C12N15/11

Abstract RU 2463347 C2

FIELD: medicine.

SUBSTANCE: characterised method provides one of standard methods for synthesis of an oligodeoxyribonucleotide sequence, introduction of a hexachlorofluoresceine-containing modifier, removal of protective groups by concentrated aqueous ammonia containing tert-butylamine in the volume relation of 2:1 to 5:1 at room temperature for 21-24 hours and recovery of an end product by one of standard methods.

EFFECT: method enables higher yield of hexachlorofluoresceine-marked oligonucleotides with the absence of acridine derivative impurities prepared in the samples regardless of the method of target oligomer recovery.

7 dwg, 4 ex

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RU 2 463 347 C2

Authors

Chuvilin Andrej Nikolaevich

Pozmogova Galina Evgen'Evna

Smirnov Igor' Pavlovich

Govorun Vadim Markovich

Dates

2012-10-10—Published

2010-05-28—Filed