METHOD FOR ASSESSING MORPHOFUNCTIONAL ACTIVITY OF EOSINOPHILS IN HELMINTHOSIS Russian patent published in 2012 - IPC G01N33/48 G01N33/49 

FIELD: medicine.

SUBSTANCE: method for assessing morphofunctional activity of eosinophils in helminthosis involves the determination of eosinophil count, an eosinophil index and an allergisation index with the additional determination of a degree of change of size and functional activity of eosinophils that is followed by the assessment of morphofunctional activity of eosinophils according to a level of specific antibody titre with the absolute eosinophil count calculated by formula wherein N is the eosinophil count calculated in a Fuchs-Rosenthal count chamber; 10 is a potency; 3.2 is the Fuchs-Rosenthal count chamber volume, the eosinophil index (EI) is calculated by formula: wherein Stab.% is a percentage of stab neutrophils; Neutr.% is a percentage of segmented neutrophils; Eos.% is a percentage of eosinophils, the assessment of a degree of allergisation in peripheral blood is calculated by the allergisation index (AI) by formula: wherein Lymph.% is a percentage of lymphocytes; Eos.% is a percentage of eosinophils; Neutr.% is a percentage of neutrophils; Mon.% is a percentage of monocytes; Bas.% is a percentage of basophils, while the degree of change of size and functional activity of eosinophils is stated by a cell contour height, increase of the relative eosinophil count, increase of the absolute eosinophil count, decrease of the specific optical density by red component and decrease of the average eosinophil diametre with a risk of helminthosis stated by increase of the cell contour height more than 400*10^-9 m - +4.3, increase of the relative eosinophil count more than 10 - +3.5, increase of the absolute eosinophil count more than 400* 10⁶/l - +3.3, decrease of the specific optical density by red component less than 0.270 - +3.3, decrease of the average eosinophil diametre less than 13.5 mcm - +2.9 and the level of specific antibody titre less than 1:800.

EFFECT: more accurate assessment of morphofunctional activity of eosinophils in helminthosis.

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