INSTRUMENT-FREE DIAGNOSTIC TECHNIQUE FOR PSEUDOTUBERCULOSIS Russian patent published in 2012 - IPC G01N33/569 G01N33/53 

FIELD: medicine.

SUBSTANCE: instrument-free diagnostic technique for pseudotuberculosis consists in the direct detection of specific antibodies in patient's blood serum by applying an antigen on a nitrocellulose membrane, drying, incubation in a blocking solution at first, then in a G-protein-carbon conjugate solution. The incubation is performed for 1 hour; the blocking solution additionally contains 0.05% Tween 20 and 1% casein; the antigen is presented by the Yersinia pseudotuberculosis protein complex. The specific anti-analyte complex is visualised for 15 minutes.

EFFECT: use of the declared method provides higher accuracy of diagnosing pseudotuberculosis.

1 ex, 2 dwg