METHOD FOR EVALUATING DEGREE OF VIRAL CYTOPATHOGENICITY Russian patent published in 2012 - IPC C12Q1/25 

FIELD: medicine.

SUBSTANCE: method involves sampling of a primary cell culture, its infection at a constant dose with strains of different cytopathogenicity, incuybation of the infected material, and evaluation of a degree of cytopathogenicity of the agent. The primary cell culture is presented by a cell suspension of peritoneal exudate of laboratory animals. A degree of viral cytopathogenicity is evaluated by variations of cytochemical enzymatic activity for the enzymes 5'-nucleotidase, ATP-ase, succinate dehydrogenase and the content of nitrogen oxide (NO) metabolites of the infected cells, in dynamics, in - 0.5,1, 2, 3, 5 and 6 h by measuring optical density of a substrate solution for each enzyme. An average cell activation value (CAV_av) is calculated by formula: CAV=CAV_0.5+CAV₁+CAV₂+CAV₃+CAV₅+CAV₆/N; wherein N is a number of time intervals. A cell response index (CRI) is calculated by formula: CRI=CAVav/ CAV intact cells 100. The average value is derived, and if the values CRI_av is less than 100, a degree of viral pathogenicity is considered to be high, while the values CRI_av more than 100 show a low degree of viral pathogenicity.

EFFECT: method provides higher efficiency, information value and reliability of evaluation of a degree of viral pathogenicity.

2 cl, 4 tbl, 2 ex