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2 468 077

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IPC

C12N1/14(2006-01-01)

C12P7/64(2006-01-01)

C12R1/645(2006-01-01)

(21) (22)

Application

2011104913/10, 2011-02-11

(24)

Start date

2011-02-11

(22)

Actual filing date

2011-02-11

(45)

Published

2012-11-27

(72)

Inventor

Sergeeva Jana EhduardovnaIvashechkin Aleksej AleksandrovichAlekhin Aleksandr IvanovichAndrijanova Diana AlekseevnaMysjakina Irina SergeevnaGal'Chenko Valerij FedorovichGalanina Ljudmila AlekseevnaFeofilova Elena PetrovnaLunin Valerij Vasil'Evich

(73)

Holder

Khimicheskij Fakul'Tet Moskovskogo Gosudarstvennogo Universiteta Imeni M.V. Lomonosova

METHOD OF PRODUCING LIPIDS Russian patent published in 2012 - IPC C12N1/14 C12P7/64 C12R1/645

Abstract RU 2468077 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Spores of filamentous fungi Cunninghamella are grown on wort agar at temperature 27-28°C for 5-6 days. Trehalose in amount of 0.2% is added to the wort agar layer. The spores are washed and held for 20-30 minutes in sterile tap water while slightly shaking. The washed off spores are fermented as inoculum for 90-96 hours at temperature 29±0.5°C on a culture medium containing glucose, ammonium nitrate, magnesium sulphate heptahydrate, potassium dihydrophosphate, a yeast extract and 0.1-0.2% D-glucosamine or 0.11-0.15% N-acetyl-B-glucosamine. The obtained biomass is separated and lyophilised. Lipids are extracted by shaking the lyophilised biomass with a mixture of chloroform and ethyl alcohol in ratio 2:1 on a shaker.

EFFECT: method increases output of lipids.

8 ex

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RU 2 468 077 C2

Authors

Sergeeva Jana Ehduardovna

Ivashechkin Aleksej Aleksandrovich

Alekhin Aleksandr Ivanovich

Andrijanova Diana Alekseevna

Mysjakina Irina Sergeevna

Gal'Chenko Valerij Fedorovich

Galanina Ljudmila Alekseevna

Feofilova Elena Petrovna

Lunin Valerij Vasil'Evich

Dates

2012-11-27—Published

2011-02-11—Filed