FIELD: medicine; microbiology.
SUBSTANCE: fermental preparation of a complex cellulase, xylanase and xyloglucanase for hydrolysis of cellulose and hemicellulose is obtained by propagation of the Penicillium verruculosum VKM F-3972D strain on the water medium containing in g/l: cellulose - 50-60, glucose - 10-30, mineral salts - KH2PO4 8-12, (NH2)2SO4 3-7, MgSO4·7H2O 0.2-0.4, CaCl2·2H2O 0.2-0.4, at continuous feeding with glucose. The propagation process is conducted at temperature of 26-30°C and pH in a range of 4.5-5.0. The culture liquid containing a fermental preparation, is separated in 120-144 h after propagation beginning, treated with ultrafiltration and dried up.
EFFECT: high specific activity of the complex of cellulase, xylanase and xyloglucanase and rising of efficiency of use of a fermental preparation in various areas of biotechnology.
2 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MYCELIAL FUNGUS PENICILLIUM FUNICULOSUM STRAIN AS PRODUCER OF CARBOHYDRASES COMPLEX CONTAINING CELLULASES, β-GLUCANASES, β-GLUCOSIDASES, XYLANASES AND XYLOGLUCANASES AND METHOD FOR PREPARING ENZYME PREPARATION OF CARBOHYDRASES COMPLEX FOR SACCHARIFICATION OF LIGNOCELULOSE MATERIALS | 2006 |
|
RU2323254C2 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
| GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE | 2013 |
|
RU2550044C2 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
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| NEW RECOMBINANT STRAIN (VERSIONS) OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM, AND ENZYMATIC AGENT (VERSIONS) INTENDED FOR HYDROLYSIS OF FRUIT RAW MATERIAL, AND METHOD OF ITS PRODUCTION | 2014 |
|
RU2574206C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION | 2017 |
|
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| STRAIN OF MYCELIAL FUNGUS ASPERGILLUS ACULEATUS AS PRODUCER OF CARBOHYDRASES COMPLEX CONTAINING XYLANASES, BETA-GLUCANASES, PECTINASES AND XYLOGLUCANASES | 2005 |
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| PENICILLIUM VERRUCULOSUM MX-73 FUNGUS STRAIN PRODUCER OF MODIFIED XYLANASE E WITH HIGH HEAT STABILITY, ENZYME PREPARATION BASED THEREON FOR USE IN FOOD AND FEED INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2018 |
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| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| STRAIN OF TRICHODERMA LONGIBRACHIATUM TW-14-220 FILAMENTOUS FUNGUS - PRODUCER OF CELLULASES, BETA-GLUCANASES AND XYLANASES FOR FEED PRODUCTION AND A METHOD FOR OBTAINING A FEED COMPLEX ENZYME PREPARATION | 2017 |
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RU2654564C1 |
