METHOD FOR DETECTION OF BOVINE BETA-LACTOGLOBULINE VERSION B* Russian patent published in 2012 - IPC C12N15/00 

FIELD: medicine.

SUBSTANCE: method provides amplification of a beta lactoglobuline gene fragment of the length of 241 base pairs (positions 1821-2061 to Z48305) which contains the mutation C→A in position 2036 to Z48305 relevant to upstream position 215 of a translation initiation site of the beta lactoglobuline gene. The fragment is modified by the introduction of a restriction site; the following analysis of reaction products is assisted by RFLP-analysis with the use of Mspl restriction nuclease. The absence of the restriction site corresponds to allele B*.

EFFECT: method enables effective and reliable diagnostics at all the stages of animal's ontogenesis that in turn makes it possible to use it in selection practice as a marker for production of low beta-lactoglobuline milk at all the stages of animal's ontogenesis.

2 dwg, 1 ex