FIELD: veterinary medicine.
SUBSTANCE: invention can be used in veterinary practice and zootechnics to diagnose kappa-casein four important alleles (κ-CNA, κ-CNB, κ-CNC, κ-CNE). Proposed is a set of primers sequences and allele-specific probes for simultaneous four mutant kappa-casein alleles gene diagnostics in cattle by performing of the real time polymerase chain reactions, having the following structure: CSN3_F tgtgctgagtaggtatcctagttatgg; CSN3_R gcgttgtcttctttgatgtctccttag; CSN3-HinfI-wt2 (FAM)-tagctactctagaagattctccaga-(RTQ1); CSN3-HinfI-m2 (R6G)-gctactctagaagcttctccagaa-(BHQ2); CSN3-HaeIII-wt (FAM)-agaagttattgagAgcccacct-(RTQ1); CSN3-HaeIII-m2 (R6G)-cagaagttattgagGgccc-(BHQ2); CSN3-MaeII-wt (FAM)-catggcacGtcacccaca-(RTQ1); CSN3-MaeII-m (R6G)-tggcacAtcacccacacc-(BHQ2).
EFFECT: simultaneous four mutant kappa-casein alleles gene diagnostics in cattle.
1 cl, 1 dwg, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR SIMULTANEOUS GENODIAGNOSTIC OF FOUR MUTANT ALLELES OF KAPPA-CASEIN IN CATTLE AND TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2017 |
|
RU2691995C2 |
| SET OF SEQUENCES OF PRIMERS AND ALLELE-SPECIFIC PROBES FOR SIMULTANEOUS GENODIAGNOSIS OF FOUR MUTANT ALLELES OF BETA-CASEIN IN CATTLE | 2022 |
|
RU2798281C1 |
| SET OF SEQUENCES OF PRIMERS AND ALLELE-SPECIFIC PROBES FOR DIFFERENTIATION OF KAPPA-CASEIN ALLELES IN CATTLE | 2020 |
|
RU2788734C2 |
| METHOD FOR SIMULTANEOUS GENE DIAGNOSIS OF FOUR ALLELES OF BETA-CASEIN IN CATTLE AND TEST SYSTEM FOR ITS IMPLEMENTATION | 2022 |
|
RU2815238C2 |
| SET OF SEQUENCE OF PRIMERS AND ALLELE-SPECIFIC PROBES FOR SIMULTANEOUS GENE DIAGNOSTICS OF TWO MUTANT ALLELES CAUSING CVM AND BLAD IN CATTLE | 2015 |
|
RU2577990C1 |
| METHOD FOR PCR WITH ALLELE-SPECIFIC PROBES FOR GENOTYPING CATTLE BY ALLELES A AND B OF THE KAPPA-CASEIN GENE | 2021 |
|
RU2791519C1 |
| METHOD OF SIMULTANEOUS GENODIAGNOSTIC OF TWO MUTANT ALLELES CAUSING CVM AND BLAD IN CATTLE, AND TEST SYSTEM FOR IT | 2015 |
|
RU2601151C2 |
| METHOD FOR DETERMINING BOVINE BETA-CASEIN GENOTYPES BY REAL-TIME PCR USING A UNIVERSAL DYE | 2022 |
|
RU2793465C1 |
| METHOD FOR CARRYING OUT REAL TIME PCR FOR GENOTYPING CATTLE ON ALLELES A AND B OF THE CSN3 GENE | 2018 |
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RU2701648C2 |
| METHOD FOR GENETIC DIAGNOSIS OF MUTANT ALLELE THAT CAUSES SHORT SPINE SYNDROME OR BRACHYSPINE SYNDROME IN CATTLE AND A TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2016 |
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RU2664454C2 |
