FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and can be used in agriculture and food industry. Constructed are recombinant nucleotide sequences which encode polypeptides (DGLA-synthase) which consist of delta-9-elongase and delta-8-desaturase, independently and separately exhibiting inherent enzymatic activity.
EFFECT: disclosed are genetic constructs containing said nucleotide sequences and host cells transformed by said constructs, preferably cells of oil plants and yeast, which express the active DGLA-synthase enzyme and can be used to obtain in said cells long-chain polyunsaturated fatty acids, particularly industrially significant dihomo-gamma-linolenic acid and eicosatetraenoic acid.
18 cl, 119 dwg, 36 tbl, 62 ex
| Title | Year | Author | Number |
|---|---|---|---|
| DELTA-5-DESATURASE AND ITS USE FOR PRODUCING POLYUNSATURATED FATTY ACIDS | 2007 |
|
RU2469092C2 |
| NOVEL DELTA-9-ELONGASE FOR PRODUCING POLYUNSATURATED FATTY ACID-ENRICHED OILS | 2010 |
|
RU2558302C2 |
| DELTA-8 DESATURASE GENES, ENZYMES ENCODED THEREBY AND USE THEREOF | 2009 |
|
RU2517615C2 |
| DNA FRAGMENT (VARIANTS), METHOD OF PLANT PREPARING, METHOD OF INDUCTION OF GAMMA-LINOLENIC ACID PRODUCING (VARIANTS), METHOD OF INDUCTION OF OCTADECATETRAENOIC ACID AND DELTA-6-DESATURASE PRODUCING IN CYANOBACTERIUM | 1992 |
|
RU2152996C2 |
| PROMOTER FOR GENE EXPRESSION AND ITS USE IN CONSTRUCTING YEAST TRANSFORMANT YARROWIA LIPOLYTICA PRODUCING β-CAROTENE | 2022 |
|
RU2819535C1 |
| DGA AND OTHER LCPUSFA PRODUCTION IN PLANTS | 2012 |
|
RU2644241C2 |
| DELTA 6-ACETYLENASE/DELTA 6-DESATURASE AND DELTA 6-DESATURASE FROM Ceratodon purpureus | 2000 |
|
RU2268939C2 |
| YARROWIA LIPOLYTICA YEAST TRANSFORMANT PRODUCING LINALOOL | 2022 |
|
RU2809554C1 |
| MODIFIED NUCLEOTIDE SEQUENCE ENCODING A POLYPEPTIDE WITH THE ACTIVITY OF PHYTOENE SYNTHASE AND GERANYLGERANYL PYROPHOSPHATE SYNTHASE (VARIANTS), ITS USE IN THE CONSTRUCTION OF CAROTENOID-PRODUCING YARROWIA LIPOLYTICA YEAST TRANSFORMANTS | 2021 |
|
RU2794980C1 |
| RECOMBINANT STRAIN YARROWIA LIPOLYTICA - PRODUCER OF ENCAPSULATED PHYTASE PAENIBACILLUS SPECIES | 2023 |
|
RU2814490C1 |
