METHOD TO STERILISE FLUID NUTRIENT MEDIA FOR CULTIVATED BIOMASS Russian patent published in 2013 - IPC C12N1/00 A61L2/00 

FIELD: biotechnologies.

SUBSTANCE: method is proposed to sterilise fluid nutrient media for cultivation of biomass. Preliminary double-staged heating of a flow of a fluid nutrient medium is carried out. Then the flow of the fluid nutrient medium is heated to temperature of sterilisation in the range from 130°C to 140°C by sending it via hollow electric heating elements for 2-6 sec. At the same time the fluid nutrient medium is maintained at the temperature of sterilisation of 130-134°C for 4-6 sec. or at the temperature of sterilisation of 136-140°C for 2-4 sec. Then the fluid nutrient medium is cooled.

EFFECT: method makes it possible to produce sterile nutrient media with high content of native sugars, makes it possible to exclude additional coolants, at the same time heat exchange equipment is simplified.

3 cl, 2 dwg, 2 ex