GOLDEN ALGAE AND METHOD FOR PRODUCTION THEREOF Russian patent published in 2013 - IPC C12N1/12 C12P23/00 

FIELD: chemistry.

SUBSTANCE: invention relates to cultivating blue-green microalgae of the genus Spirulina to obtain golden yellow algae with high content of carotenoids. The method involves cultivating algae of the genus Spirulina in a culture medium containing 0.1-0.15 g/l of a compound which contains carbon as a carbon source and 0.06-0.35 g/l of a compound which contains nitrogen as a nitrogen source, for 5-7 days. Further, concentration of the carbon source is increased from 0.2 g/l to 1.5 g/l and concentration of the nitrogen source is reduced from 0.005 g/l to 0.03 g/l and cultivation continues for the next 5-7 days. A citrate compound is then periodically added to the culture medium in amount of 0.5-10 mg/l or an acetate compound is periodically added in amount of 75-250 mg/l or combinations thereof are added. The obtained golden yellow microalgae of the genus Spirulina, having high content of carotenoids, are collected.

EFFECT: obtained algae contain at least 600 mg/g of dry cell mass of mixed carotenoids.

19 cl, 6 tbl, 1 ex