METHOD FOR ESTIMATING CLINICAL COURSE OF WOUND PROCESS AND CLINICAL EFFECTIVENESS IN SKIN WOUNDS OF VARIOUS GENESES Russian patent published in 2013 - IPC G01N33/483 

FIELD: medicine.

SUBSTANCE: what is presented is a method for estimating clinical course of the wound process and clinical effectiveness of skin wounds of various geneses. A wound surface is scraped to recover DNA by means of a DNA-EXPRESS reagent with an electrophoretic detection stain; the prepared supernatant containing DNA is introduced in a well 4 mm long with 2% agarous gel with ethydium bromide and exposed to horizontal electrophoresis for 15 minutes. The derived electrophoretograms are used to measure the length of the formed tracks. If the track length exceeds the well length in 4.7-5.6 times and more, the phase I wound process is diagnosed. If the track length exceeds the well length in 3.8-4.6 times and more, the phase II wound process is diagnosed. If the track length exceeds the well length in 2.6-3.7 times and more, the phase III wound process is diagnosed. The track length tending to shorten with the developing wound process is estimated as the positive clinical course of wound healing, and the therapy is considered to be effective; track lengthening and no length variation are estimated as the negative clinical course of wound healing with the therapy to be ineffective, and the therapeutic approach to be changed.

EFFECT: invention enables estimating the clinical effectiveness in skin wounds over a short period of time and choosing a therapeutic approach correctly, prescribing remedies and methods of treating, and timely correcting the therapeutic intervention.

5 dwg, 2 tbl, 5 ex