FIELD: agriculture.
SUBSTANCE: invention relates to plant physiology and biochemistry. First wheat seeds are germinated in 0.004 mM of sodium butyrate solution, at specified time intervals from beginning of soaking 0h, 3h, 6h, 9h, 12h, 15h, 18h, 21h, separation of embryos from endosperm is performed with further conservation of embryos in buffered 80-90% glycerol at minus 25°C, cell nuclei are isolated, extraction of nuclear fractions by growing concentrations 0.14 M and 0.35 M, 2 M of sodium chloride and 6 M of guanidine hydrochloride with 0.1% β-mercaptoethanol is performed and proteolytic and inhibiting activity are determined in said nuclear fractions.
EFFECT: invention allows to estimate impact of protein deacetilation inhibitor on induction of plant growth morphogenesis.
1 ex, 1 tbl, 4 dwg
