APPLICATION OF PROTEIN HYDROLYSATES FOR STABILISATION OF DETERGENT COMPOSITIONS OF METALLOPROTEASE Russian patent published in 2013 - IPC C12N9/96 C11D3/38 C11D3/386 

FIELD: biotechnologies.

SUBSTANCE: composition of neutral metalloprotease stabilised with an inhibitor is proposed to produce a liquid detergent solution. The composition contains from approximately 0.001% to approximately 10% wt of neutral metalloprotease and a competitive inhibitor. Besides, the competitive inhibitor represents a protein hydrolysate and is connected with at least approximately 90% of molecules of the specified neutral metalloprotease. Also the method is proposed to produce a composition of neutral metalloprotease stabilised with an inhibitor. The mixture is incubated, which contains at least one neutral metalloprotease and a protein substrate, in the water buffer at pH in the range from approximately 6.5 to approximately 11 and at the temperature from approximately 22°C to approximately 37°C. In process of incubation the substrate protein is split when exposed to metalloprotease, which results in formation of the hydrolysis product. The hydrolysis product is extracted with molecular weight of less than approximately 5000 Da and combined with neutral metalloprotease.

EFFECT: higher stability of detergent compositions during storage without reduction of desired activity of neutral metalloprotease in process of application.

12 cl, 3 dwg, 5 tbl, 3 ex