PROTEASES COMPRISING ONE OR MORE COMBINABLE MUTATIONS Russian patent published in 2015 - IPC C12N15/09 C12N15/00

Abstract RU 2560978 C2

FIELD: chemistry.

SUBSTANCE: present invention relates to biotechnology and is an isolated version of Bacillus subtilisin, where said subtilisin version is a mature form having subtilisin activity and comprising a substitution at positions 118 and 213, where the numbering of the positions corresponds to the amino acid sequence of B. Amyloliquefaciens subtilisin BPN′ with the sequence SEQ ID NO: 1,where said Bacillus subtilisin is FNA, and where said subtilisin version further comprises combinations of substitutions. The invention also relates to a method of cleaning fabric using said subtilisin version.

EFFECT: invention enables to obtain subtilisin versions with a higher protein expression level.

5 cl, 5 dwg, 4 tbl, 4 ex

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RU 2 560 978 C2

Authors

Kaskao-Perejra, Luis G.

Ehstell, Dehvid A.

Kellis, Ml., Dzhejms T.

Dates

2015-08-20—Published

2009-11-10—Filed