PRODUCTION OF THERMOLYSIN AND ITS VERSIONS, AND ITS USE IN LIQUID DETERGENTS Russian patent published in 2014 - IPC C12N9/54 C11D3/386 

FIELD: biotechnologies.

SUBSTANCE: invention proposes a version of thermolysin, which has an improved efficiency in comparison to thermolysin of a wild type. The above version of thermolysin contains a replacement chosen from T006G, F063P, T006H, S065K, T006I, S065Y, T006K, Y075G, T006M, Y075M, T006N, Y075T, T006P, Q128H, T006Q, Q128I, T006R, Q128L, T006V, Q128M, T006W, Q128V, T006Y, Q128Y, V007F, Y151D, V007H, Y151E, V007K, Y151H, V007L, Y151K, V007M, Y151M, V007P, Y151N, V007Q, Y151Q, V007R, Y151R, V007T, Y151T, V007Y, Y151V, T049G, T049H, I156M, T049I, I156R, T049K, I156T, T049L, I156W, T049N, G196R, T049P, Q273I, T049Q, Q273P, T049W, Q273Y, A058I, T278K, A058P, T278M, A058R, T278P, F063I, N280K, F063L, N280R. Thermolysin is obtained by transformation of Bacillus sp. with an expression vector containing a polynucleotide sequence coding a version of thermolysin and by cultivation of a transformed host cell under conditions suitable for production of the above thermolysin. This ferment is used as a part of a cleaning composition for cleaning of surfaces or items.

EFFECT: obtaining a version of thermolysin with improved efficiency.

55 cl, 9 dwg, 3 tbl, 8 ex