METHOD OF PRODUCING COPOLYMER OF 3-HYDROXYBUTYRIC ACID AND 3-HYDROXYVALERIC ACID Russian patent published in 2013 - IPC C12P7/62 C12N1/20 C12R1/05 

FIELD: chemistry.

SUBSTANCE: method of producing copolymer of 3-hydroxybutyric acid and 3-hydroxyvaleric acid involves culturing a Ralstonia eutropha All-Russian collection of industrial microorganisms V-5786 producer strain in aeration conditions and mixing on a liquid salt medium which contains a hydrogen- and carbon-containing growing substrate with addition of a salt of valeric acid. The strain is cultured in two steps while continuously feeding a nitrogen source for 24-36 hours at the first step and without nitrogen at the second step. The growing substrate used is a gaseous mixture of H₂, CO₂, CO and O₂, wherein O₂ is fed into the culture in a separate stream while continuously feeding potassium valerate into the culture.

EFFECT: high total output of the copolymer.

1 tbl, 3 ex