PRODUCING TERMINAL ALKENES VIA ENZYMATIC DECARBOXYLATION OF 3-HYDROXYALKANOIC ACIDS Russian patent published in 2015 - IPC C12P5/02 C12N9/88 C12N15/60 C12N15/63 C12N1/20 C12N1/21 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to biotechnology. The invention discloses a method of producing a terminal alkene, in a particular version - isobutylene, use of mevalonate diphosphate decarboxylase and a microorganism which produces mevalonate diphosphate decarboxylase to obtain a terminal alkene, as well as a composition for producing a terminal alkene. The method includes converting 3-hydroxyalkanoate of formula using mevalonate diphosphate decarboxylase in the presence of ATP, rNTP, a dNTP or a mixture of several of such molecules, a polyphosphate or pyrophosphate into a terminal alkene. R¹ and R² are independently selected from a group consisting of a hydrogen atom and a straight or branched alkyl group which includes 1-4 carbon atoms. Isobutylene is obtained by converting 3-hydroxy-3-methylbutyrate using mevalonate diphosphate decarboxylase which includes SEQ ID NO:6, or a sequence having at least 15%, preferably 50%, 80% or 90% sequence homology. A composition for producing a terminal alkene includes a microorganism which produces mevalonate diphosphate decarboxylase, a culture medium with a carbon source and 3-hydroxyalkanoate.

EFFECT: invention provides a new way for synthesis of a terminal alkene based on enzymatic conversion of 3-hydroxyalkanoates, which enables to prevent use of petroleum products and lowers the cost of producing plastics and fuel.

25 cl, 15 dwg, 13 ex