METHOD OF DETECTING LYSINE IN MIXTURE OF α-AMINO ACIDS Russian patent published in 2013 - IPC G01N33/15 

FIELD: chemistry.

SUBSTANCE: method of detecting lysine in a mixture of α-amino acids, except a mixture containing cysteine and proline, involves preparation of an acetate-ammonia buffer solution with pH 3.06 to which is added 0.1% lysine solution in such an amount that its content in the final solution ranges from 0.0004 to 0.008%; 0.2% aqueous ninhydrin solution in such an amount that content of ninhydrin in the final solution ranges from 0.01 to 0.03%; heating the obtained solution on a water bath at 95-105°C for 15-25 minutes; adding cetylpyridinium chloride in an amount which ensures its content in the solution in a concentration after critical micelle concentration of 0.1-0.4%; measuring the absorption spectrum of the obtained solution; detecting lysine in the solution based on the presence of a maximum at wavelength λ_max=480 nm.

EFFECT: simpler, faster and cheaper procedure of determining lysine while maintaining high metrological parameters of the determining method.

1 tbl, 11 dwg