METHOD OF IDENTIFICATION AGENT BASED ON HIGH THROUGHPUT SCREENING Russian patent published in 2013 - IPC C12N15/10 C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: method comprises the following stages: preparing cells in the first set comprising at least 96 receivers, adding an agent at least to one receiver; incubating the agent with the cells, lysing the cells by adding a buffer which is hypotonic or comprises detergent, and contains an inhibitor of DNAase; removal of RNA from the cell lysate with ribonuclease; centrifugation of the first set of receivers at low speed; transfer of a supernatant containing DNA fragments to the second set of receivers; adding a detectable compound capable of intercalation with DNA fragments in a buffer for detection containing DNAase inhibitor to at least one receiver; measuring the amount of intercalated detectable compound; and comparing the amount of intercalated detectable compound to the control for determining the difference that enables to identify the said agent as a modifying agent if the difference exceeds a predetermined limit.

EFFECT: invention enables to detect agents that affect the formation of DNA fragments in cells using high performance screening applications.

10 cl, 14 dwg, 2 tbl, 11 ex