FIELD: biotechnology, immunology. SUBSTANCE: hybridoma is prepared by immunization of hamster with a rat purified αd and rat transfected αd CHO cells followed by fusion of splenocytes with NS-1 myeloma cells and screening the target cells. Hybridoma produces monoclonal antibody exhibiting reaction with α-subunit of rat integrin αd. Invention can be used in immunohistochemical analysis for assay of localization of αd. Invention ensures to determine localization of αd in tissues. EFFECT: improved method of hybridoma preparing, valuable properties. 2 cl, 4 dwg, 1 tbl
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