FIELD: chemistry.
SUBSTANCE: material cells are mechanically disintegrated; lipids are extracted using a Folch method, involving extraction of lipids with a mixture of chloroform and methanol (2:1 by volume), followed by washing the extract with KCl solution (0.88%) and, after demixing and removing the top phase, with a mixture of 0.88% KCl solution and methanol (1:1 by volume); the KCl solution and the mixture are added in an amount of 1/4 part of the obtained and remaining volume of extract, respectively; the ready lipid extract is dried by passing through a layer of anhydrous Na2SO4; the dry extract is then re-esterified with a mixture of methanol and acid catalyst, where the methanol is mixed with the catalyst in ratio of 50:1 by volume; biodiesel is extracted from the reaction mixture with hexane; the biodiesel extract is dried by passing through a layer of anhydrous Na2SO4.
EFFECT: obtaining biodiesel using a cheap and simple method by processing mud or sludge from treatment facilities.
1 tbl, 2 ex
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Authors
Dates
2013-07-20—Published
2011-11-24—Filed