PERMANENT CELL LINE OF SIBERIAL STURGEON (Acipenser baeri) FINS USED FOR VIRUSOLOGICAL RESEARCH OF FISH Russian patent published in 2013 - IPC C12N5/71 C12N7/00 

FIELD: biotechnologies.

SUBSTANCE: presented cell line is produced from primarily trypsinized tissue of Siberial sturgeon fins by means of long-term passivation in the medium 199 with Hanks salts with 10% embryonal serum and is deposited in the Russian Academy of Agricultural Sciences (agricultural animals) under the No.76. The line may be used in laboratory research in production of virus vaccines and diagnostic preparations for extraction, accumulation, titration and study of a virus haemorrhagic septicemia virus (VHSV) of salmon fishes, infectious haemopoetic necrosis (IHNV) of salmon fish tissue, spring viremia of carps (SVCV), carp iridovirus (CCIV) and herpes of sturgeon virus (SbSHV).

EFFECT: cell line has a high coefficient of passage, which makes it possible to accumulate high number of biomaterial for a short period of time, and is a promising heterogenerous system for cultivation of viruses from fishes of non-sturgeon breeds, preventing development of non-specific reactions due to absence of antibodies to cell proteins.