METHOD TO PRODUCE DNA FROM SALMON ROE Russian patent published in 2013 - IPC C12P19/34 C07H21/04 

Abstract RU 2488634 C1

FIELD: biotechnologies.

SUBSTANCE: desoxyribonucleic acid (DNA) is extracted from salmon roe. Used raw materials are defrosted and ground. They are treated with aqueous solution of tripsin for 3 hours at 45°C with the following content of components (wt %): salmon roe - 40.0, tripsin - 0.02-0.22, water - balance. The produced hydrolysate is centrifuged and filtered via belting cloth. Lyophilic or spray drying is carried out. Yield of finished products from salmon roe makes 10.0-18.0% of the quantity of initial raw materials with DNA content in the target product equal to at least 62 - 65%.

EFFECT: increased yield of produced DNA.

1 tbl, 5 ex

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RU 2 488 634 C1

Authors

Albulov Aleksej Ivanovich

Frolova Marina Alekseevna

Samujlenko Anatolij Jakovlevich

Vasil'Chenko Ol'Ga Mikhajlovna

Shinkarev Sergej Mikhajlovich

Grin' Andrej Vladimirovich

Rogov Roman Vasil'Evich

Nemtsev Dmitrij Sergeevich

Dates

2013-07-27Published

2012-01-20Filed