FIELD: biotechnologies.
SUBSTANCE: invention includes developed medium formulations for cultivation of human fibroblasts in presence of low (2%) concentration of serum of xenogenic, allogenic and autogenic (autologous) origin.
EFFECT: reduced variability of composition of substantial growth factors, risk of contamination with cell pathogens, reduced level of media cytotoxicity and their potential immunogenicity; creation of adequate low-serum media for maintennace and growth media for solutionof various tasks of diploid strains and tissue cultures cultivation; development of the possibility to use an autogenic serum for cultivation of cells and tissues of a donor with no risks of infectious contamination, cytotoxicity and immune conflict.
2 cl, 5 dwg, 4 ex
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