METHOD OF OBTAINING LIVE CULTURAL ATTENUATED VACCINE FOR THE PREVENTION OF VARICELLA Russian patent published in 2017 - IPC A61K39/25 C12N7/04 A61P31/22 

FIELD: medicine.

SUBSTANCE: method of obtaining live cultural attenuated vaccine for the prevention of varicella includes infection with varicella-zoster virus strain of OKA diploid cells LEC-3 with the subsequent reproduction and the release of virus into cultural medium. Thereafter, at least one collection of the virus-containing liquid, the addition of a stabiliser and the preparation of the vaccine are carried out. The group of inventions also relates to the use of a strain of diploid LEC-3 cells for the production of a live cultural attenuated vaccine for the prevention of varicella. The use of diploid cells of strain LEC-3 with high adhesiveness to the surface of growth allows to obtain from 7 to 15 individual virus collections with a high content of extracellular vaccine Varicella zoster virus.

EFFECT: obtaining extracellular virus Varicella zoster by individual collections from infected diploid cells LEC-3 using trisodium citrate provides increased harvest of Varicella zoster virus than in similar cultural models when used as a dispersant of trypsin.

7 cl, 1 tbl, 3 dwg