METHOD FOR LOCAL WOUND HEALING BY MEANS OF BIOLOGICAL DRESSING CONTAINING LIVE CELLS OF HUMAN DIPLOID FIBROBLASTS Russian patent published in 2014 - IPC A61L15/00 A61K35/48 A61K38/39 A61P17/02 A61P41/00 

Abstract RU 2526811 C1

FIELD: medicine.

SUBSTANCE: method for local wound healing involving using a biological dressing which is applied on a wound surface. The biological dressing contains a polymer base of a hydrophobic perforated silicone film coated with a layer of human collagen type I and human diploid cells. The biological dressing is square-shaped and contains the diploid cells in the form of the characterised live cells of M-20 human fibroblasts at the level of passages No. 20-33 in the form of cell monolayers of 70-80% saturation density prepared at a starting density of (4-5)×104 cells per 1 cm2 of the dressing and culturing in a nutrient medium with fibrinolytically active plasma added. Vast injuries may require placing a desired number of the line-on-line dressings on the wound surface. Preferentially, the dressing is applied from the 1st-2nd post-injury day.

EFFECT: improving repair processed in the wound and reducing time of healing.

4 cl, 4 ex, 1 tbl, 3 dwg

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Authors

Khubutija Mogeli Shalvovich

Mikhajlov Mikhail Ivanovich

Ozherelkov Sergej Viktorovich

Konjushko Ol'Ga Ivanovna

Smirnov Sergej Vladimirovich

Zhirkova Elena Aleksandrovna

Storozheva Majja Viktorovna

Makarov Maksim Sergeevich

Borovkova Natal'Ja Valer'Evna

Mironov Aleksandr Sergeevich

Khvatov Valerij Borisovich

Dates

2014-08-27Published

2013-07-09Filed