FIELD: medicine.
SUBSTANCE: method for local wound healing involving using a biological dressing which is applied on a wound surface. The biological dressing contains a polymer base of a hydrophobic perforated silicone film coated with a layer of human collagen type I and human diploid cells. The biological dressing is square-shaped and contains the diploid cells in the form of the characterised live cells of M-20 human fibroblasts at the level of passages No. 20-33 in the form of cell monolayers of 70-80% saturation density prepared at a starting density of (4-5)×104 cells per 1 cm2 of the dressing and culturing in a nutrient medium with fibrinolytically active plasma added. Vast injuries may require placing a desired number of the line-on-line dressings on the wound surface. Preferentially, the dressing is applied from the 1st-2nd post-injury day.
EFFECT: improving repair processed in the wound and reducing time of healing.
4 cl, 4 ex, 1 tbl, 3 dwg
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Authors
Dates
2014-08-27—Published
2013-07-09—Filed