METHOD TO DETECT NUMBER OF MICROORGANISMS IN AIR Russian patent published in 2013 - IPC C12Q1/02 G01N27/06 

FIELD: biotechnologies.

SUBSTANCE: 1% sterile solution of glucose is prepared on the basis of a physiological solution, which is used as a nutrient medium. An absorber by Zaytsev is connected to an aspirator of "Briz-1" grade, into the flask of which 10 ml of the prepared 1% glucose solution is placed. The device is placed into the investigated room, and the aspirator is switched on for 15 min. Microorganisms that are in air go through the glucose solution and are retained in it. The solution is placed into a test tube and thermostatted at 37°C for 2 hours. Solution electroconductivity is measured with the help of a sensor KDS-1038. The number of microorganisms in air is determined according to the curve of empirical dependence of solution electroconductivity on the number of microbes, which is built in accordance with the produced values.

EFFECT: invention makes it possible to reduce time for determination of microorganism number in the air of the working area down to 2 hours and 20 min.

1 dwg