FIELD: biotechnologies.
SUBSTANCE: method provides for growing of a culture of microscopic fungi on a dense nutrient medium. A standard suspension containing 104CFU/ml is prepared from the grown culture of microscopic fungi. The suspension in the amount of 0.5 ml is seeded into 4.5 ml of molten agar nutrient medium cooled down to 45°C. Mixed, poured into a Petri cup to produce a seeded plate of the agar nutrient medium. The seeded agar plate is cut into blocks with the specified size, which are placed onto the microscope slide and covered with the cover slop with formation by the agar chamber - production of the preparation. The produced preparation is incubated in the moist chamber at 28°C for 3 weeks.
EFFECT: invention makes it possible to simplify methodology of preparations making and to expand area of application of the produced preparations.
3 dwg, 2 ex
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Authors
Dates
2013-10-27—Published
2012-05-22—Filed