METHOD OF CULTIVATION OF MICROALGAE FOR BIOFUEL PURPOSE Russian patent published in 2013 - IPC C12N1/12 C12Q1/00 

FIELD: biotechnology.

SUBSTANCE: method of cultivation of microalgae for biofuel purpose comprises two stages of algolisation. At the first stage the algolisation is carried out by primary inoculum of the culture, preferably Chlorella vulgaris BIN, obtained in the photobioreactor synchronously or time-shifted, of multifunctional indoor pools with translucent railing. The total volume of the said pools is from 1/30 to 1/15 of the total amount of open water reservoirs. The secondary inoculum is grown with a bulk density of 10⁹-10¹¹ cells/l, at that the cultivation is started in spring with an average daily temperature of water in the pools in the range of 12-18°C. The second stage of cultivation the microalgae is started by selection from the pools of secondary inoculum at a temperature of water in the open water reservoirs of 12-18°C, and continue its supply to the open water reservoirs to reach in them of the bulk density of microalgae 2·10⁸-10⁹. The secondary inoculum is partially taken from the pool as a finished product in spring and autumn with the water temperature in them is in the range of 8-12°C, at that in multifunctional pools the equal amount of water with dissolved biogens is added. The resulting microalgae contain 33.9 % cellulose, 51.0 % proteins, 7.3% fats.

EFFECT: increased productivity of microalgae cultivation method.

3 cl, 1 dwg, 4 ex