FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents a method for semi-industrial cultivation of red microalgae Porphyridium purpureum, relates to biotechnology of microalgae, namely to intensive cultivation of red sea microalgae Porphyridium purpureum, and is intended for production of its biomass enriched with red pigment B-phycoerythrin. Method comprises growing microalgae P. purpureum in open cultivators located in a greenhouse module, with working layer thickness of 10 cm for 13 days in autumn-winter period and for 8 days in spring period at natural seasonal level of illumination and temperature, with continuous stirring of the culture with an aquarium pump, on a nutrient medium based on sea water with the addition of sea salt to concentration of 28 g⋅l-1, having the following composition: NaNCO3 – 1.2 g⋅l-1, NaH2PO4×2H2O – 0.45 g⋅l-1, EDTA-Na2 – 0.037 g⋅l-1, FeC6H5O7×3H2O – 0.0265 g⋅l-1, MnCl2×4H2O – 0.004 g⋅l-1, Co(NO3)2×6H2O – 0.0031 g⋅l-1, (NH4)6Mo7O24×4H2O – 0.0009 g⋅l-1, K2Cr2(SO4)2×4H2O – 0.0017 g⋅l-1.
EFFECT: invention enables to obtain Porphyridium purpureum biomass of the required quality with preservation of high rate of culture growth and increase average annual productivity of microalgae cultivation systems in greenhouse complexes.
1 cl, 3 dwg, 1 tbl
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Authors
Dates
2025-01-10—Published
2024-05-20—Filed