FIELD: chemistry.
SUBSTANCE: invention refers to biotechnology. A method is claimed for extraction of lipids from microalgae of Chlorella genus and yeast Yarrowia lipolytica for producing biodiesel fuel. The method includes supplementation of the culturing stage of microalgae Chlorella with culturing stage of yeast Yarrowia lipolytica W23. Lipid fraction is extracted from the microalgae and yeast biomass using organic solvents chloroform - methanol in ratio 2:1-1:2, the residual microalgae biomass and residual yeast biomass being hydrolyzed at a temperature of 80-140°C for 20-90 minutes, and this biomass in the form of aqueous suspension is used as a nutrient medium for culturing the yeast Yarrowia lipolytica W23.
EFFECT: invention provides for high lipid output: up to 37% based on dry weight.
6 cl, 1 dwg, 1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF SELECTING LIPIDES FROM BIOMASS OF MICROWAVES OF THE GENUS Chlorella | 2015 |
|
RU2617959C1 |
| METHOD FOR CHEMICAL MODIFICATION OF MICROALGAE LIPIDS, METHOD OF PRODUCING SOAP AND SOAP CONTAINING FATTY ACID SALTS OF SAPONIFIED MICROALGAE LIPIDS | 2009 |
|
RU2542374C2 |
| METHOD OF PRODUCING PEPTIDE FRACTION FROM WATER-SOLUBLE PROTEINS OF MICROALGAE, HAVING ANTIBIOTIC PROPERTIES | 2023 |
|
RU2824212C1 |
| PROCESS FOR PREPARING BIOLOGICALLY ACTIVE SUBSTANCES FROM BIOMASS OR CHLORELLA MICROALGA | 1992 |
|
RU2044770C1 |
| METHOD OF PRODUCING A PIGMENT COMPLEX FROM THE BIOMASS OF UNICELLULAR ALGAE OF CHLORELLA GENUS | 2018 |
|
RU2695879C1 |
| METHOD FOR CULTIVATION OF MICROALGAE CHLORELLA KESSLERI FOR USE AS BIOCOMPONENT OF FUEL | 2023 |
|
RU2819445C1 |
| METHOD OF EXTRACTING LIPIDS FROM MICROALGA CHLORELLA SOROKINIANA | 2018 |
|
RU2694405C1 |
| METHOD FOR OBTAINING BIOMASS OF MICROALGAE CHLORELLA VULGARIS | 2022 |
|
RU2797012C1 |
| METHOD OF CULTIVATION OF BIOMASS WITH INCREASED CONTENT OF LIPIDS | 2014 |
|
RU2569149C1 |
| METHOD FOR PRODUCTION OF FATTY ACIDS METHYL ESTER SUITABLE FOR USE IN ENGINE | 2012 |
|
RU2603748C2 |
