FIELD: biotechnologies.
SUBSTANCE: method for obtaining spore material of bacteria of Clostridium type provides for production of inoculum of bacteria in a full synthetic growth medium, seeding of inoculum and cultivation under the corresponding conditions in growth medium including potato, glucose, ammonium sulphate and chalk. During the main fermentation process for 18-28 parts of bacterial culture growth depending on time of occurrence in one field of view of at least 80-100 thickened forms of cells, n-butanol in the amount of 0.16-0.81 wt % is added to growth medium. Number of formed spores is 1.08-2.86·108 in one millilitre of the medium.
EFFECT: increased content of spores in spore material.
1 tbl, 15 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF BACTERIUM CLOSTRIDIUM ACETOBUTYLICUM - A PRODUCER OF NORMAL BUTYL ALCOHOL AND ACETONE | 1995 |
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| METHOD OF OBTAINING BUTANOL | 2008 |
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| CLOSTRIDIUM ACETOBUTYLICUM BACTERIA STRAIN - PRODUCER OF N-BUTYL ALCOHOL, ACETONE AND ETHANOL | 2008 |
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| METHOD FOR MICROBIOLOGICAL SYNTHESIS OF N-BUTANOL | 2009 |
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| CLOSTRIDIUM ACETOBUTYLICUM STRAIN - N-BUTANOL PRODUCER | 2011 |
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| METHOD OF PREPARING ORGANIC SOLVENTS, APPARATUS FOR REALISING SAID METHOD, PRODUCT OBTAINED USING SAID METHOD | 2008 |
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| METHOD OF PRODUCING ORGANIC SOLVENTS, MAINLY BUTANOL | 2008 |
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RU2375454C1 |
