SET OF SYNTHETIC OLIGONUCLEOTIDES FOR IDENTIFICATION OF DNA OF PARODONTOPATHOGENIC MICROORGANISM Treponema denticola BY METHOD OF POLYMERASE CHAIN REACTION Russian patent published in 2014 - IPC C12Q1/68 C12N15/11 

Abstract RU 2510857 C2

FIELD: chemistry.

SUBSTANCE: invention relates to field of biochemistry, in particular to set of synthetic oligonucleotides for identification of DNA of parodontopathogenic microorganism Treponema denticola by method of polymerase chain reaction. Claimed set includes specific to fragment of gene licCA of microorganism Treponema denticola primers 5'-TAG CCG GAA AAA CGA AGG AGT G-3' and 5'-CCC TGC TTG TTT GCA AAC ATA G-3', as well as probe (BHQ1)-5'-AAC CCA GCC G(FdT)T TCG TCC TCC GAC-3'-P, where BHQ1 stands for attached to 5'-tail nucleotide blank fluorescence damper, FdT - fluorescent dye FAM, attached to T nucleotide.

EFFECT: claimed invention represents an effective marker for identification of microorganism Treponema denticola presence in biological material.

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Authors

Rebrikov Denis Vladimirovich

Zorina Oksana Aleksandrovna

Petrukhina Natalija Borisovna

Boriskina Ol'Ga Andreevna

Berkutova Irina Sergeevna

Ajmadinova Nelli Kamil'Evna

Dates

2014-04-10Published

2012-02-24Filed