FIELD: chemistry.
SUBSTANCE: invention relates to biochemistry, particularly a synthetic oligonucleotide set for detecting DNA of periodontal-pathogenic microorganism Tannerella forsythensis via a polymerase chain reaction. Said set includes Tannerella forsythensis gene bspA fragment specific primers 5'-TGGCACCCTCCGATGCCGAC-3' and 5'-GCGCAGACCGTTGGGTTTCA-3', as well as a probe (BHQ1)-5'-CCGCGCCCGA(FdT)GCGTCGCTGGC-3'-P, wheree BHQ1 denotes a dark fluorescence extinguisher attached to the 5'-terminal nucleotide, and FdT is a fluorescent dye FAM attached to the nucleotide T.
EFFECT: present invention provides reliable detection of presence of Tannerella forsythensis in biological material.
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Authors
Dates
2014-12-20—Published
2012-02-24—Filed