FIELD: veterinary medicine.
SUBSTANCE: method of cryoconservation of rooster semen is characterised in that the rooster semen is diluted 5 times in a medium which contains 100 cm3 of distilled water, 6 g of lactose, 0.58 g of citric acid, 1 g of tris-oxymethyl-aminomethane, 10 cm3 of hen egg yolk, 5 cm3 of glycerin, while the temperature of the semen and the medium is 18-20°C. The diluted semen is maintained for 2 hours in the refrigerator at a temperature of 2-5°C and then frozen as pellets with the volume of 0.2 ml on the fluoroplastic plate cooled to liquid nitrogen temperature and placed in liquid nitrogen.
EFFECT: use of the claimed invention enables to enhance the protective qualities of the medium for cryopreservation of semen and to increase the quality characteristics of frozen-thawed semen of roosters.
1 tbl
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SU1706608A1 |
Authors
Dates
2014-06-10—Published
2012-11-09—Filed